Dataset on inflammatory proteins expressions and sialic acid levels in apolipoprotein E-deficient mice with administration of N-acetylneuraminic acid and/or quercetin

Rongrong Dong; Fahui Li; Shucun Qin; Yi Wang; Yanhong Si; Xuelian Xu; Hua Tian; Lei Zhai; Guangjie Zhang; Yongjun Li; Yawei Zhou; Ying Zhang; Nan Zhang; Shoudong Guo

doi:10.1016/j.dib.2016.06.020

Dataset on inflammatory proteins expressions and sialic acid levels in apolipoprotein E-deficient mice with administration of N-acetylneuraminic acid and/or quercetin

Data Brief. 2016 Jun 22:8:613-7. doi: 10.1016/j.dib.2016.06.020. eCollection 2016 Sep.

Authors

Rongrong Dong¹, Fahui Li², Shucun Qin¹, Yi Wang³, Yanhong Si¹, Xuelian Xu⁴, Hua Tian¹, Lei Zhai¹, Guangjie Zhang¹, Yongjun Li¹, Yawei Zhou¹, Ying Zhang¹, Nan Zhang¹, Shoudong Guo¹

Affiliations

¹ Key Laboratory of Atherosclerosis in Universities of Shandong Province, Institute of Atherosclerosis, Taishan Medical University, Taian 271000, China.
² Department of Chemistry and Chemical Engineering, Weifang University, Weifang 261061, China.
³ Department of Ophthalmology, Affilated Hospital of Taishan Medical University, Taian 271000, China.
⁴ School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

Abstract

The data presented in this article describe an effect of N-acetylneuraminic acid and/or quercetin on the inflammatory proteins expressions (TNF-α, ICAM-1, VCAM-1 and MOMA-2) and the N-acetylneuraminic acid (NANA) levels of apolipoprotein E-deficient mice that are given a high-fat diet. Protein expression was performed by immunohistochemical imaging and NANA was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) or semi-quantified using Image-Pro Plus software after ligation with fluorescein-5-thiosemicarbazide (FTSC). Further interpretation and discussion could be found at our research article entitled "Exogenous supplement of N-acetylneuraminic acid ameliorates atherosclerosis in apolipoprotein E-deficient mice" (Guo et al., 2016) [1].

Keywords: Fluorescent labeling; Imaging; Immunohistochemistry; LC-MS/MS; Sialic acid.