Role of vitamin D3 in regulation of interleukin-6 and osteopontin expression in liver of diabetic mice

Eur Rev Med Pharmacol Sci. 2016 Jul;20(13):2916-9.


Objective: To study the link between hepatic interleukin-6 (IL-6) and osteopontin (OPN) gene expression and vitamin D3 status associated with type 1 diabetes in mice; and to evaluate the effects of vitamin D3 treatment (800 IU/kg of body weight for 6 weeks) on diabetes-induced impairments.

Materials and methods: mRNA levels of IL-6 and OPN were measured by quantitative RT-PCR. Blood serum 25OHD3 was assayed by ELISA.

Results: It was shown that induction of IL-6 in diabetic liver is accompanied by increased expression of OPN. Changes in OPN and IL-6 RNA levels correlated with a lack of 25OHD3 in serum. Vitamin D3 treatment restored 25OHD3 that led to a substantial reduction of OPN and IL-6 mRNA levels.

Conclusions: Diabetes-induced vitamin D3 deficiency was associated with increased hepatic levels of IL-6 and OPN mRNA and these changes were countered by vitamin D3 administration.

MeSH terms

  • Animals
  • Cholecalciferol / metabolism*
  • Cholecalciferol / pharmacology
  • Diabetes Mellitus, Experimental / metabolism*
  • Diabetes Mellitus, Type 1 / genetics
  • Diabetes Mellitus, Type 1 / metabolism*
  • Gene Expression Regulation / drug effects*
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Liver / drug effects
  • Liver / metabolism
  • Mice
  • Osteopontin / genetics
  • Osteopontin / metabolism*


  • Interleukin-6
  • Osteopontin
  • Cholecalciferol