Modulation of endothelial cell expression of intercellular adhesion molecule 1 by protein kinase C activation

Biochem Biophys Res Commun. 1989 Jun 30;161(3):945-52. doi: 10.1016/0006-291x(89)91334-x.

Abstract

Inflammatory stimuli enhance the adherence properties of human umbilical vein endothelial cells (HUVEC) for neutrophils (PMN). This is mediated in part by the up-regulation on HUVEC of Intercellular Adhesion Molecule 1 (ICAM 1). Phorbol esters, which activate protein kinase c (PKC), have also been reported to enhance the adherence properties of HUVEC for PMN. We investigated the effect of agents which activate PKC on the expression of ICAM 1 by HUVEC. Both phorbol myristate acetate (PMA) and Mezerein, a non-phorbol which also stimulates PKC, enhanced both the expression of ICAM 1 on HUVEC and the adherence of HUVEC for PMN. The PKC inhibitors staurosporine and H-7 prevented both PMA and Mezerein-induced stimulation of HUVEC expression of ICAM 1 and adherence for PMN. We conclude that activation of PKC in HUVEC is associated with increased expression of ICAM 1 on HUVEC. PKC-mediated up-regulation of ICAM 1 may be responsible, in part, for the promotion of endothelial cell adherence properties toward PMN.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Alkaloids / pharmacology
  • Antigens, Surface / analysis*
  • Carcinogens / pharmacology
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Diterpenes*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Enzyme Activation
  • Humans
  • Isoquinolines / pharmacology
  • Kinetics
  • Membrane Glycoproteins / biosynthesis*
  • Phorbol Esters / pharmacology
  • Piperazines / pharmacology
  • Protein Kinase C / metabolism*
  • Staurosporine
  • Terpenes / pharmacology
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Alkaloids
  • Antigens, Surface
  • Carcinogens
  • Cell Adhesion Molecules
  • Diterpenes
  • Isoquinolines
  • Membrane Glycoproteins
  • Phorbol Esters
  • Piperazines
  • Terpenes
  • phorbol-12,13-dibenzoate
  • mezerein
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Protein Kinase C
  • Staurosporine
  • Tetradecanoylphorbol Acetate