Chamigrane Sesquiterpenes from a Basidiomycetous Endophytic Fungus XG8D Associated with Thai Mangrove Xylocarpus granatum

Mar Drugs. 2016 Jul 15;14(7):132. doi: 10.3390/md14070132.

Abstract

Six new chamigrane sesquiterpenes, merulinols A‒F (1‒6), and four known metabolites (7‒10) were isolated from the culture of the basidiomycetous fungus XG8D, a mangrove-derived endophyte. Their structures were elucidated mainly by 1D and 2D NMR, while the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. The in vitro cytotoxicity of all compounds was evaluated against three human cancer cell lines, MCF-7, Hep-G2, and KATO-3. Compounds 3 and 4 selectively displayed cytotoxicity against KATO-3 cells with IC50 values of 35.0 and 25.3 μM, respectively.

Keywords: chamigrane; cytotoxic; endophytic fungus; sesquiterpene.

MeSH terms

  • Basidiomycota / chemistry*
  • Cell Line, Tumor
  • Crystallography, X-Ray / methods
  • Drug Screening Assays, Antitumor / methods
  • Endophytes / chemistry*
  • Fungi / chemistry*
  • Hep G2 Cells
  • Humans
  • MCF-7 Cells
  • Molecular Structure
  • Rhizophoraceae / microbiology*
  • Sesquiterpenes / chemistry*
  • Sesquiterpenes / pharmacology
  • Thailand
  • X-Ray Diffraction / methods

Substances

  • Sesquiterpenes