Two new bioactive iridoids from Rothmannia wittii

Fitoterapia. 2016 Sep:113:97-101. doi: 10.1016/j.fitote.2016.07.007. Epub 2016 Jul 16.

Abstract

The first reported study of the isolation and identification of compounds from the bark and fruit of Rothmannia wittii yielded two new iridoids, 6β-hydroxy-10-O-acetylgenipin (1) and 10-O-acetylmacrophyllide (2) together with six known iridoids; 6β-hydroxygenipin (3), genipin (4), garjasmine (5), cerbinal (6), and mixture of β-gardiol (7) and α-gardiol (8); benzoic acid (9); vanillic acid (10); and stigmasterol (11). Their structures were elucidated by spectroscopic methods. Iridoid 1 showed antimycobacterial activity against Mycobacterium tuberculosis with a MIC value of 12.50μg/mL. Iridoid 2 showed cytotoxicity against the NCI-H187 cancer cell line with an IC50 value of 6.82μg/mL. In addition, 2 and 5 exhibited weak cytotoxic activity against KB and MCF-7 cell lines, while 4 was active against the NCI-H187 cancer cell line.

Keywords: Cerbinal; Garjasmine; Genipin; Iridoid; Macrophyllide; Rothmannia wittii.

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / isolation & purification
  • Antineoplastic Agents, Phytogenic / chemistry*
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Cell Line, Tumor
  • Fruit / chemistry
  • Humans
  • Iridoids / chemistry*
  • Iridoids / isolation & purification
  • Molecular Structure
  • Mycobacterium tuberculosis / drug effects*
  • Plant Bark / chemistry
  • Rubiaceae / chemistry*

Substances

  • Anti-Bacterial Agents
  • Antineoplastic Agents, Phytogenic
  • Iridoids