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Case Reports
. 2016 Aug;22(8):1485-7.
doi: 10.3201/eid2208.151962.

Human Tick-Borne Encephalitis and Characterization of Virus From Biting Tick

Free PMC article
Case Reports

Human Tick-Borne Encephalitis and Characterization of Virus From Biting Tick

Anna J Henningsson et al. Emerg Infect Dis. .
Free PMC article


We report a case of human tick-borne encephalitis (TBE) in which the TBE virus was isolated from the biting tick. Viral growth and sequence were characterized and compared with those of a reference strain. Virus isolation from ticks from patients with TBE may offer a new approach for studies of epidemiology and pathogenicity.

Keywords: RNA; case report; cell culture; flavivirus; sequence analysis; tick-borne encephalitis; tick-borne encephalitis virus; vector-borne infections; viruses.


Figure 1
Figure 1
Time course of tick-borne encephalitis (TBE) in a 67-year-old man in Sweden, 2011. A classic biphasic onset of symptoms is shown. PHC, primary healthcare center.
Figure 2
Figure 2
Time course of tick-borne encephalitis virus (TBEV) multiplication from sample from a 67-year-old man in Sweden, 2011. A549 cells were infected with the virus isolated from the tick in this study, tick/SWE/Habo/2011/1, and reference strain Hypr at multiplicity of infection 0.1. Total cellular RNA and cell culture supernatants were collected at different time points postinfection. A) Intracellular levels of viral RNA quantified by real-time reverse transcription PCR analysis. B) Virus titers in cell culture supernatants as determined by focus-forming assay (FFU). Mean values from 3 independent experiments are shown; error bars indicate SD. p values determined by Student t test.

Comment in

  • Human Tick-Borne Encephalitis, the Netherlands.
    Hira V, Rockx B. Hira V, et al. Emerg Infect Dis. 2017 Jan;23(1):169. doi: 10.3201/eid2301.161405. Epub 2017 Jan 15. Emerg Infect Dis. 2017. PMID: 27938522 Free PMC article. No abstract available.

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