Feruloyl esterase production by Aspergillus terreus CECT 2808 and subsequent application to enzymatic hydrolysis

N Pérez-Rodríguez; C D Moreira; A Torrado Agrasar; J M Domínguez

doi:10.1016/j.enzmictec.2016.05.011

Feruloyl esterase production by Aspergillus terreus CECT 2808 and subsequent application to enzymatic hydrolysis

Enzyme Microb Technol. 2016 Sep:91:52-8. doi: 10.1016/j.enzmictec.2016.05.011. Epub 2016 Jun 1.

Authors

N Pérez-Rodríguez¹, C D Moreira², A Torrado Agrasar³, J M Domínguez⁴

Affiliations

¹ Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.
² Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain; CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
³ Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain.
⁴ Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, SPAIN and Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain. Electronic address: jmanuel@uvigo.es.

PMID: 27444329
DOI: 10.1016/j.enzmictec.2016.05.011

Abstract

Ferulic acid esterases (FAE) were produced by Aspergillus terreus CECT 2808 from vine trimming shoots (VTS) and corn cob. Later, the fungal extracts thus obtained were used to enzymatically release ferulic acid (FA) from both substrates. Our findings showed a higher FAE activity in the enzymatic extracts produced on corn cob (0.070±0.004U/mL). Nevertheless, the enzymatic extracts produced on VTS demonstrated a better performance for FA release from both corn cob (2.05±0.01mg/g) and VTS (0.19±0.003mg/g). This result was probably because of the higher xylanase/FAE ratio determined in VTS extract. Therefore, an additional assay was carried out by supplementing corn cob extract with a commercial xylanase to test the influence of FAE/xylanase ratio in FA release. The results revealed the relevance of the FAE/xylanase ratio for an optimal FA release.

Keywords: Corn cob; Enzymatic hydrolysis; Ferulic acid; Feruloyl esterase; Vine trimming shoot.

MeSH terms

Aspergillus / enzymology*
Carboxylic Ester Hydrolases / metabolism*
Coumaric Acids / metabolism
Endo-1,4-beta Xylanases / metabolism
Fermentation
Fungal Proteins / metabolism*
Hydrolysis
Lignin / metabolism
Substrate Specificity
Vitis / chemistry
Zea mays / chemistry

Substances

Coumaric Acids
Fungal Proteins
lignocellulose
Lignin
ferulic acid
Carboxylic Ester Hydrolases
feruloyl esterase
Endo-1,4-beta Xylanases