Holographic Assessment of Lymphoma Tissue (HALT) for Global Oncology Field Applications

Theranostics. 2016 Jun 18;6(10):1603-10. doi: 10.7150/thno.15534. eCollection 2016.


Low-cost, rapid and accurate detection technologies are key requisites to cope with the growing global cancer challenges. The need is particularly pronounced in resource-limited settings where treatment opportunities are often missed due to the absence of timely diagnoses. We herein describe a Holographic Assessment of Lymphoma Tissue (HALT) system that adopts a smartphone as the basis for molecular cancer diagnostics. The system detects malignant lymphoma cells labeled with marker-specific microbeads that produce unique holographic signatures. Importantly, we optimized HALT to detect lymphomas in fine-needle aspirates from superficial lymph nodes, procedures that align with the minimally invasive biopsy needs of resource-constrained regions. We equipped the platform to directly address the practical needs of employing novel technologies for "real world" use. The HALT assay generated readouts in <1.5 h and demonstrated good agreement with standard cytology and surgical pathology.

Keywords: Cancer diagnostics; Point-of-care diagnostics; Smartphone testing; global oncology..

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy, Fine-Needle
  • Holography / methods*
  • Lymph Nodes / pathology
  • Lymphoma / diagnosis*
  • Lymphoma / pathology
  • Microspheres
  • Molecular Diagnostic Techniques / methods*
  • Optical Imaging / methods*
  • Smartphone*
  • Staining and Labeling / methods*
  • Time Factors