Perinatal lethality (ple): a mutation caused by integration of a transgene into distal mouse chromosome 15

Genomics. 1989 May;4(4):498-504. doi: 10.1016/0888-7543(89)90272-3.


We have used cytogenetic and recombinational analysis to determine the position of a transgene integrated into the mouse genome. The transgene maps to band F on the physical map of mouse chromosome 15 by in situ analysis and is tightly linked genetically to a cluster of loci that include the mutations caracul (Ca) and microcytic anemia (mk). Genetic analysis of the offspring of noninbred animals carrying the transgene and marker loci demonstrates a significant deficiency of homozygous progeny at weaning. When inbred mice heterozygous for the transgene are mated, about one-quarter of their offspring are homozygous; none of these animals survives more than 1 day after birth. It appears likely that a recessive insertional mutation has occurred as a result of transgene integration into a locus required for postnatal viability. We call this mutation transgenic perinatal lethality (Tg.ple).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosome Mapping
  • Genes, Lethal*
  • Genes, Recessive
  • Genes, Synthetic
  • Mice
  • Mice, Inbred Strains / genetics
  • Mice, Transgenic / genetics*
  • Mutation
  • Nucleic Acid Hybridization
  • Oncogenes
  • Recombination, Genetic