Abstract
Human oral spirochetes are prominent inhabitants of subgingival plaque in patients with periodontal disease. By immunoelectron microscopy using protein A-gold complexes and either polyclonal mouse antiserum against the 53-kDa antigen or 53-kDa-antigen-specific monoclonal antibody, a major polypeptide antigen, with a molecular weight of 53,000 (molecular size, 53 kilodaltons [kDa]), of a human oral spirochete, Treponema denticola ATCC 33520, was found to localize on the surface of the outer envelope.
MeSH terms
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Agglutination Tests
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Antibodies, Bacterial
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Antibodies, Monoclonal
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Antibody Specificity
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Antigens, Bacterial / immunology
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Antigens, Bacterial / isolation & purification*
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Antigens, Surface / immunology
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Antigens, Surface / isolation & purification*
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Humans
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Immunohistochemistry
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Microscopy, Electron
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Periodontal Diseases / microbiology
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Periodontal Diseases / pathology
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Treponema / immunology*
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Treponema / ultrastructure
Substances
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Antibodies, Bacterial
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Antibodies, Monoclonal
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Antigens, Bacterial
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Antigens, Surface