Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

F A Rubin; P D McWhirter; N H Punjabi; E Lane; P Sudarmono; S P Pulungsih; M Lesmana; S Kumala; D J Kopecko; S L Hoffman

doi:10.1128/jcm.27.5.1112-1114.1989

Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

J Clin Microbiol. 1989 May;27(5):1112-4. doi: 10.1128/jcm.27.5.1112-1114.1989.

Authors

F A Rubin¹, P D McWhirter, N H Punjabi, E Lane, P Sudarmono, S P Pulungsih, M Lesmana, S Kumala, D J Kopecko, S L Hoffman

Affiliation

¹ Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.

Abstract

A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Autoradiography
DNA Probes*
DNA, Bacterial / analysis*
Humans
Nucleic Acid Hybridization
Salmonella typhi / genetics
Salmonella typhi / isolation & purification*
Sepsis / diagnosis
Sepsis / microbiology*
Typhoid Fever / diagnosis
Typhoid Fever / microbiology*

Substances

DNA Probes
DNA, Bacterial