Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics

Proteomics. 2016 Oct;16(20):2718-2731. doi: 10.1002/pmic.201600141. Epub 2016 Sep 12.


The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established the proteome profiles on nude mice skin to verify the specific responses during aging caused by different factors. Our results showed that UVB-elicited aging results in upregulation of proliferating cell nuclear antigen and strong oxidative damage in DNA, whereas chronological aging abolished epidermal cell growth and increased the expression of caspase-14, as well as protein carbonylation. Network analysis indicated that the programmed skin aging activated the ubiquitin system and triggered obvious downregulation of 14-3-3 sigma, which might accelerate the loss of cell growth capacity. On the other hand, UVB stimulation enhanced inflammation and the risk of skin carcinogenesis. Collectively, functional proteomics could provide large-scale investigation of the potent proteins and molecules that play important roles in skin subjected to both intrinsic and extrinsic aging.

Keywords: Animal Proteomics; Chronological; Network analysis; Proteome; UVB; Ubiquitin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / analysis
  • 14-3-3 Proteins / metabolism
  • Aging
  • Animals
  • Cell Differentiation / radiation effects
  • Cell Proliferation / radiation effects
  • Female
  • Mice, Nude
  • Oxidative Stress / radiation effects
  • Protein Interaction Maps*
  • Proteome / analysis
  • Proteome / metabolism*
  • Proteomics
  • Skin / chemistry
  • Skin / metabolism*
  • Skin / radiation effects
  • Skin / ultrastructure
  • Skin Aging*
  • Ubiquitins / analysis
  • Ubiquitins / metabolism
  • Ultraviolet Rays


  • 14-3-3 Proteins
  • Proteome
  • Sfn protein, mouse
  • Ubiquitins