Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq

Genome Med. 2016 Jul 27;8(1):80. doi: 10.1186/s13073-016-0335-7.

Abstract

Accurate characterization of the repertoire of the T-cell receptor (TCR) alpha and beta chains is critical to understanding adaptive immunity. Such characterization has many applications across such fields as vaccine development and response, clone-tracking in cancer, and immunotherapy. Here we present a new methodology called single-cell TCRseq (scTCRseq) for the identification and assembly of full-length rearranged V(D)J T-cell receptor sequences from paired-end single-cell RNA sequencing reads. The method allows accurate identification of the V(D)J rearrangements for each individual T-cell and has the novel ability to recover paired alpha and beta segments. Source code is available at https://github.com/ElementoLab/scTCRseq .

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity
  • Animals
  • Gene Expression Regulation
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor*
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor*
  • Humans
  • Jurkat Cells
  • Mice
  • RNA, Messenger / genetics*
  • RNA, Messenger / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Receptors, Antigen, T-Cell, alpha-beta / immunology
  • Sequence Analysis, RNA / methods*
  • Single-Cell Analysis / methods*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology
  • V(D)J Recombination

Substances

  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta

Associated data

  • GEO/GSE45428