Response to Comment on "A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme"

Science. 2016 Jul 22;353(6297):358. doi: 10.1126/science.aad6398.

Abstract

Wang et al report a failure to reproduce our biochemical observation that the INO80C and SWR1C/SWR1/SWR-C chromatin remodeling enzymes catalyze replacement of nucleosomal H2A.Z with H2A when the substrate contains H3-K56Q. They point to technical problems with our dimer exchange assay. In response, we have recapitulated our findings using a mobility shift assay that was developed and employed by Wang and colleagues.

Publication types

  • Research Support, N.I.H., Extramural
  • Comment

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Chromatin Assembly and Disassembly*
  • Histones / metabolism*
  • Multienzyme Complexes / metabolism*
  • Nucleosomes / metabolism*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Histones
  • Multienzyme Complexes
  • Nucleosomes
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphatases