Objective: Members of the transient receptor potential (TRP) channels are involved in mediating the electrical excitability and stimulus-secretion coupling in the pancreatic β-cells. The expression and the relative abundance of different TRP channels in the human β-cells are unknown. The objective of this study was to examine the expression of the TRP channels and their relative abundance in the human β-cell.
Methods: RNA sequencing data obtained from human islets, fluorescence-activated cell sorting-purified human β-cell and human pancreatic acinar cells were analyzed. Gene counts and fragments per kilobase per million mapped reads were obtained.
Results: Among the TRPC family only the TRPC1 was expressed in the human β-cell. TRPV1 channels were not expressed in the human β-cells. Among the TRPM family, TRPM4, TRPM7, TRPM2, and TRPM3 were expressed in the human β-cell. Of the remaining TRP channels, TRPP2, TRPML1, and TRPML3 were expressed in these cells.
Conclusions: By analyzing the RNA sequencing data, we have detected for the first time the TRP channels that are expressed in the purified human β-cells, in comparison to the other relevant pancreatic cell types. Our study provides an opportunity to focus on these TRP channels for a better understanding of the electrophysiology and stimulus-secretion coupling in these cells.