Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags

Biomarkers. 2017 Mar;22(2):113-122. doi: 10.1080/1354750X.2016.1210678. Epub 2016 Jul 28.


Context and objective: Human hepatocellular carcinoma (HCC) is a severe malignant disease, and accurate and reliable diagnostic markers are still needed. This study was aimed for the discovery of novel marker candidates by quantitative proteomics.

Methods and results: Proteomic differences between HCC and nontumorous liver tissue were studied by mass spectrometry. Among several significantly upregulated proteins, translocator protein 18 (TSPO) and Ras-related protein Rab-1A (RAB1A) were selected for verification by immunohistochemistry in an independent cohort. For RAB1A, a high accuracy for the discrimination of HCC and nontumorous liver tissue was observed.

Conclusion: RAB1A was verified to be a potent biomarker candidate for HCC.

Keywords: Proteomics; RAB1A; TSPO; immunohistochemistry; liver cancer; tandem mass tags.

MeSH terms

  • Biomarkers, Tumor / analysis
  • Carcinoma, Hepatocellular / diagnosis*
  • Humans
  • Liver Neoplasms / diagnosis*
  • Proteome / analysis*
  • Proteomics / methods
  • Tandem Mass Spectrometry*
  • Up-Regulation
  • rab1 GTP-Binding Proteins / analysis


  • Biomarkers, Tumor
  • Proteome
  • rab1 GTP-Binding Proteins