Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos

Mol Biochem Parasitol. 1989 Jul;35(3):277-83. doi: 10.1016/0166-6851(89)90214-4.

Abstract

High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amanitins / pharmacology
  • Animals
  • Ascaris / genetics*
  • Brugia / genetics*
  • Cell-Free System
  • Cloning, Molecular
  • DNA-Directed RNA Polymerases / genetics*
  • Female
  • Nucleic Acid Hybridization
  • RNA Polymerase III / genetics*
  • RNA, Ribosomal, 5S / biosynthesis
  • Terminator Regions, Genetic
  • Transcription, Genetic*
  • Xenopus / genetics

Substances

  • Amanitins
  • RNA, Ribosomal, 5S
  • DNA-Directed RNA Polymerases
  • RNA Polymerase III