The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model

Biomed Res Int. 2016;2016:8091353. doi: 10.1155/2016/8091353. Epub 2016 Jul 11.

Abstract

Objective. The aim of this study was to analyze the effects of the HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b(+)Gr1(+) cells using the K14E7 transgenic mouse model. Materials and Methods. The morphology of DCs was analyzed in male mouse skin on epidermal sheets using immunofluorescence and confocal microscopy. Flow cytometry was used to determine the percentages of DCs and CD11b(+)Gr1(+) cells in different tissues and to evaluate the migration of DCs. Results. In the K14E7 mouse model, the morphology of Langerhans cells and the migratory activity of dendritic cells were abnormal. An increase in CD11b(+)Gr1(+) cells was observed in the blood and skin of K14E7 mice, and molecules related to CD11b(+)Gr1(+) chemoattraction (MCP1 and S100A9) were upregulated. Conclusions. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of DCs and induces the systemic accumulation of CD11b(+)Gr1(+) cells.

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • CD11b Antigen / metabolism*
  • Cell Count
  • Cell Movement
  • Cell Proliferation
  • Cell Shape
  • Dendritic Cells / metabolism*
  • Dendritic Cells / pathology
  • Dendritic Cells / virology*
  • Epidermis / pathology
  • Hyperplasia
  • Lectins, C-Type / metabolism
  • Lymph Nodes / pathology
  • Male
  • Mannose-Binding Lectins / metabolism
  • Mice, Transgenic
  • Models, Animal
  • Papillomavirus E7 Proteins / metabolism*
  • Spleen / pathology

Substances

  • Antigens, Surface
  • CD11b Antigen
  • Cd207 protein, mouse
  • Lectins, C-Type
  • Mannose-Binding Lectins
  • Papillomavirus E7 Proteins
  • oncogene protein E7, Human papillomavirus type 16