Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 11 (6), 900-1

A Shape Shifting Organelle: Unusual Mitochondrial Phenotype Determined With Three-Dimensional Electron Microscopy Reconstruction

Affiliations

A Shape Shifting Organelle: Unusual Mitochondrial Phenotype Determined With Three-Dimensional Electron Microscopy Reconstruction

Eugenia Trushina. Neural Regen Res.

Figures

Figure 1
Figure 1
Mitochondrial morphology in the hippocampal brain tissue of APP/PS1 mouse visualized with 2D TEM and 3D EM reconstruction. (A) Transmission electron microscopy (TEM) of the brain tissue (0.1 μm thick) revealed mitochondria in neuropils as multiple individual teardrop shaped organelles. Asterisks define parts of mitochondria-on-a-string (MOAS) that could be visible only using 3D EM reconstruction. Scale bar: 1 μm. (B) 3D EM reconstruction of the same brain tissue as in A done using 40 consecutive brain slices (0.1 μm thick) revealed a predominant MOAS phenotype.
Figure 2
Figure 2
Conditions associated with energy failure including hypoxia or advanced age induce mitochondria-on-a-string (MOAS) formation in the brain tissue of wide-type (WT) mice. (A) Exposure of a WT mouse 10 weeks of age to CO2 for 5 minutes produced MOAS observed by transmission electron microscopy (TEM) of the hippocampal brain tissue (serial section 0.1 μm thick). Scale bar: 2 μm. (B) MOAS in the hippocampus in a WT mouse 88 weeks of age sacrificed by cervical dislocation. Scale bar: 1 μm. * Depicts MOAS connections visible using 2D TEM; # depicts parts of MOAS that could be accurately defined only using 3D EM reconstruction.

Similar articles

See all similar articles

Cited by 1 PubMed Central articles

References

    1. Chang CR, Blackstone C. Dynamic regulation of mitochondrial fission through modification of the dynamin-related protein Drp1. Ann N Y Acad Sci. 2010;1201:34–39. - PMC - PubMed
    1. Dhingra R, Kirshenbaum LA. Regulation of mitochondrial dynamics and cell fate. Circ J. 2014;78:803–810. - PubMed
    1. Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell. 2013;153:1194–1217. - PMC - PubMed
    1. Mishra N, Kar R, Singha PK, Venkatachalam MA, McEwen DG, Saikumar P. Inhibition of mitochondrial division through covalent modification of Drp1 protein by 15 deoxy-Delta(12, 14)-prostaglandin J2. Biochem Biophys Res Commun. 2010;395:17–24. - PMC - PubMed
    1. Rabinovici GD, Furst AJ, Alkalay A, Racine CA, O’Neil JP, Janabi M, Baker SL, Agarwal N, Bonasera SJ, Mormino EC, Weiner MW, Gorno-Tempini ML, Rosen HJ, Miller BL, Jagust WJ. Increased metabolic vulnerability in early-onset Alzheimer's disease is not related to amyloid burden. Brain. 2010;133:512–528. - PMC - PubMed
Feedback