Modulation of LINE-1 Retrotransposition by a Human SAMHD1 Polymorphism

Virol Rep. 2016 Dec;6:53-60. doi: 10.1016/j.virep.2016.06.001.

Abstract

The HIV-1 restriction factor SAMHD1 has the ability to negatively modulate retrotransposition of the long interspersed element 1(LINE-1). By exploring the ability of human SAMHD1 polymorphisms to inhibit LINE-1, we found that the single nucleotide polymorphism S33A present in the Korean population lose the ability to inhibit LINE-1 retrotransposition. Because SAMHD1 residue S33 is phosphorylated in human cycling and non-cycling cells, we demonstrated that SAMHD1 requires to be either phosphorylated on position 33 or to contain a bulky residue in order to inhibit LINE-1 retrotransposition. Therefore this unique mutation uncouples functions in this important restriction factor.

Keywords: HIV-1; LINE-1; SAMHD1; SNPs; restrcition; retrotransposition.