Comparison of glycosaminoglycan chemical exchange saturation transfer using Gaussian-shaped and off-resonant spin-lock radiofrequency pulses in intervertebral disks

Magn Reson Med. 2017 Jul;78(1):280-284. doi: 10.1002/mrm.26362. Epub 2016 Aug 3.

Abstract

Purpose: To investigate, if a train of spin-lock pulses (chemical exchange saturation transfer with spin-lock pulses = CESL) improves biochemical glycosaminoglycan imaging compared with conventional chemical exchange saturation transfer with Gaussian-shaped pulses (CEST) in lumbar intervertebral discs.

Methods: T2 , CEST, and CESL imaging was performed in lumbar intervertebral discs of 15 healthy volunteers at 3 Tesla. Mean and standard deviation of the asymmetric magnetization transfer ratio (MTRasym ), the asymmetric spin-lock ratio (SLRasym ) and T2 values were calculated for nucleus pulposus (NP) and annulus fibrosus (AF). Wilcoxon test was used to analyze differences between MTRasym and SLRasym . Pearson correlation was used to determine the relationship between MTRasym , SLRasym and T2 .

Results: Data showed no significant difference between MTRasym and SLRasym (NP: P = 0.35; AF: P = 0.34). MTRasym and SLRasym values differed significantly between NP and AF (MTRasym : P = 0.014, SLRasym : P = 0.005). T2 values correlated significantly with MTRasym (NP: ρ = 0.76, P < 0.001; AF: ρ = 0.60, P < 0.001) and SLRasym (NP: ρ = 0.73, P < 0.001; AF: ρ = 0.47, P < 0.001).

Conclusion: CESL does not improve the chemical exchange asymmetry effect compared with conventional CEST, but leads to comparable results. Magn Reson Med 78:280-284, 2017. © 2016 International Society for Magnetic Resonance in Medicine.

Keywords: chemical exchange saturation transfer; glycosaminoglycans; intervertebral disks; spin-lock.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Computer Simulation
  • Data Interpretation, Statistical
  • Glycosaminoglycans / metabolism*
  • Humans
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Intervertebral Disc / diagnostic imaging*
  • Intervertebral Disc / metabolism*
  • Magnetic Resonance Imaging / methods*
  • Middle Aged
  • Models, Statistical
  • Molecular Imaging / methods*
  • Normal Distribution
  • Radio Waves
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted*
  • Spin Labels
  • Young Adult

Substances

  • Glycosaminoglycans
  • Spin Labels