Specific Amplification of a DNA Sequence Common to All Chlamydia Trachomatis Serovars Using the Polymerase Chain Reaction

Res Microbiol. 1989 Jan;140(1):7-16. doi: 10.1016/0923-2508(89)90053-3.

Abstract

Enzymatic DNA amplification was applied to DNA and elementary bodies of C. trachomatis. Oligonucleotide primers were chosen in a sequence of a conserved domain of the major outer membrane protein to generate the amplification of a 129-base pair fragment. This sequence was amplified in the 15 serovars of C. trachomatis; however, serovar J gave a weaker signal than the others. The specificity was controlled by EcoRI restriction enzyme digestion and Southern analysis using an internal probe of the amplified sequence. No cross-reaction was shown with DNA of 11 other bacteria. Thus, enzymatic DNA amplification by the polymerase chain reaction appears to be a potential tool for the specific detection of C. trachomatis.

MeSH terms

  • Base Sequence
  • Chlamydia trachomatis / classification
  • Chlamydia trachomatis / genetics*
  • DNA, Bacterial / genetics*
  • Deoxyribonuclease EcoRI / metabolism
  • Gene Amplification
  • Oligonucleotide Probes
  • Species Specificity

Substances

  • DNA, Bacterial
  • Oligonucleotide Probes
  • Deoxyribonuclease EcoRI