N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

FEBS Lett. 2016 Sep;590(18):3168-78. doi: 10.1002/1873-3468.12341. Epub 2016 Sep 12.


N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

Keywords: N-glycosylation; Sulfolobus acidocaldarius; archaea; dolichol; thermoacidophile.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Archaeal Proteins / metabolism*
  • Dolichol Phosphates / chemistry
  • Dolichol Phosphates / metabolism*
  • Glycosylation
  • Protein Processing, Post-Translational*
  • Sulfolobus acidocaldarius / metabolism*


  • Archaeal Proteins
  • Dolichol Phosphates