Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry

Methods Mol Biol. 2016;1460:337-43. doi: 10.1007/978-1-4939-3810-0_23.

Abstract

Mitochondrial function is a key component of skeletal muscle health, and its dysfunction has been associated with a wide variety of diseases. Microplate-based respirometry measures aerobic respiration of live cells through extracellular changes in oxygen concentration. Here, we describe a methodology to measure aerobic respiration of intact murine skeletal muscle tissue. The tissues are not cultured, permeabilized, or enzymatically dissociated to single fibers, so there is minimal experimental manipulation affecting the samples prior to acquiring measurements.

Keywords: Aerobic respiration; Mitochondria; Oxygen consumption rate; Seahorse; Skeletal muscle.

MeSH terms

  • Animals
  • Cell Respiration / physiology*
  • Energy Metabolism
  • Mice
  • Mitochondria, Muscle / metabolism
  • Muscle, Skeletal / physiology*
  • Oxygen Consumption