Co-production of carbonic anhydrase and phycobiliproteins by Spirulina sp. and Synechococcus nidulans

Joana da Costa Ores; Marina Campos Assumpção de Amarante; Susana Juliano Kalil

doi:10.1016/j.biortech.2016.07.133

Co-production of carbonic anhydrase and phycobiliproteins by Spirulina sp. and Synechococcus nidulans

Bioresour Technol. 2016 Nov:219:219-227. doi: 10.1016/j.biortech.2016.07.133. Epub 2016 Jul 30.

Authors

Joana da Costa Ores¹, Marina Campos Assumpção de Amarante¹, Susana Juliano Kalil²

Affiliations

¹ Universidade Federal do Rio Grande, Escola de Química e Alimentos, PO Box 474, Rio Grande, RS 96203-900, Brazil.
² Universidade Federal do Rio Grande, Escola de Química e Alimentos, PO Box 474, Rio Grande, RS 96203-900, Brazil. Electronic address: dqmsjk@furg.br.

PMID: 27494103
DOI: 10.1016/j.biortech.2016.07.133

Abstract

The aim of this work was to study the co-production of the carbonic anhydrase, C-phycocyanin and allophycocyanin during cyanobacteria growth. Spirulina sp. LEB 18 demonstrated a high potential for simultaneously obtaining the three products, achieving a carbonic anhydrase (CA) productivity of 0.97U/L/d and the highest C-phycocyanin (PC, 5.9μg/mL/d) and allophycocyanin (APC, 4.3μg/mL/d) productivities. In the extraction study, high extraction yields were obtained from Spirulina using an ultrasonic homogenizer (CA: 25.5U/g; PC: 90mg/g; APC: 70mg/g). From the same biomass, it was possible to obtain three biomolecules that present high industrial value.

Keywords: Allophycocyanin; C-phycocyanin; Cell disruption; Enzyme production; Microalgae.

MeSH terms

Biomass
Carbonic Anhydrases / biosynthesis*
Phycobiliproteins / biosynthesis*
Spirulina / metabolism*
Synechococcus / metabolism*

Substances

Phycobiliproteins
Carbonic Anhydrases