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. 2016;1476:15-21.
doi: 10.1007/978-1-4939-6361-4_2.

Direct PCR-Ribotyping of Clostridium Difficile

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Direct PCR-Ribotyping of Clostridium Difficile

Sandra Janezic. Methods Mol Biol. .

Abstract

PCR-ribotyping, a method based on heterogeneity of ribosomal intergenic spacer region, is the preferred method for genotyping of Clostridium difficile. Standardly used procedure for PCR-ribotyping is culturing of C. difficile from fecal samples and subsequent typing. In this chapter, we describe a modified PCR-ribotyping method for direct detection of PCR-ribotypes directly in total stool DNA extract, without prior need to isolate C. difficile.

Keywords: Clostridium difficile; Direct typing; PCR-ribotyping; Total stool DNA.

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