The Correlation of Increased CRP Levels with NFKB1 and TLR2 Polymorphisms in the Case of Morbid Obesity

Scand J Immunol. 2016 Nov;84(5):278-283. doi: 10.1111/sji.12471.

Abstract

Morbid obesity (MO) is associated with an increase in circulating levels of systemic acute phase proteins such as C-reactive protein (CRP). Toll-like receptor is possible candidate for inflammatory responses which is mainly mediated by NFKB1. The aim of this study was to investigate the relationship between NFKB1 and Toll-like receptor (TLR) 2 polymorphisms and the risk of MO in a Turkish population in the context of CRP serum levels which may contribute to susceptibility to the disease. We analysed the distribution of NFKB1-94 ins/del ATTG rs28362491 and TLR2 Arg753Gln rs5743708 polymorphisms using PCR-RFLP method and CRP serum levels using ELISA method in 213 MO and 200 healthy controls. The frequency of the ins/ins genotype and ins allele of rs28362491 was significantly higher in the patients compared to control group (P: 0.0309; P: 0.0421, respectively). Additionally, the frequency of GG genotype and G allele of rs5743708 was found to be statistically higher in the patient group (P: 0.0421; P < 0.0001, respectively). In addition, serum CRP levels (>20 mg/l) in MO patients with ins/ins genotype were significantly higher than in patients with del/ins genotype (P: 0.0309). Serum CRP levels were also higher in MO patients with GG genotype and G allele (P: 0.0001). According to combined analysis, the wild type of rs28362491 and rs5743708 polymorphisms (ins/ins/GG genotype) was also significantly higher in the patient group versus the control group when compared with the combined ins/ins/GA and del/ins/GA genotype (P < 0.0001). Therefore, our findings suggest that rs28362491 and rs5743708 polymorphisms were significantly associated with MO disease through acting by modulating serum CRP levels.

MeSH terms

  • Adult
  • Alleles
  • C-Reactive Protein / genetics*
  • C-Reactive Protein / immunology
  • Case-Control Studies
  • Female
  • Gene Expression
  • Gene Frequency
  • Genetic Predisposition to Disease*
  • Haplotypes
  • Humans
  • Male
  • Middle Aged
  • NF-kappa B p50 Subunit / genetics*
  • NF-kappa B p50 Subunit / immunology
  • Obesity, Morbid / diagnosis
  • Obesity, Morbid / genetics*
  • Obesity, Morbid / immunology
  • Odds Ratio
  • Polymorphism, Genetic*
  • Toll-Like Receptor 2 / genetics*
  • Toll-Like Receptor 2 / immunology

Substances

  • NF-kappa B p50 Subunit
  • NFKB1 protein, human
  • TLR2 protein, human
  • Toll-Like Receptor 2
  • C-Reactive Protein