Functional engineered human cardiac patches prepared from nature's platform improve heart function after acute myocardial infarction

Biomaterials. 2016 Oct;105:52-65. doi: 10.1016/j.biomaterials.2016.07.035. Epub 2016 Jul 29.

Abstract

With the advent of induced pluripotent stem cells and directed differentiation techniques, it is now feasible to derive individual-specific cardiac cells for human heart tissue engineering. Here we report the generation of functional engineered human cardiac patches using human induced pluripotent stem cells-derived cardiac cells and decellularized natural heart ECM as scaffolds. The engineered human cardiac patches can be tailored to any desired size and shape and exhibited normal contractile and electrical physiology in vitro. Further, when patching on the infarct area, these patches improved heart function of rats with acute myocardial infarction in vivo. These engineered human cardiac patches can be of great value for normal and disease-specific heart tissue engineering, drug screening, and meet the demands for individual-specific heart tissues for personalized regenerative therapy of myocardial damages in the future.

Keywords: Cardiac patch; Cardiomyocytes; Decellularized heart matrix; Engineering human heart tissue; Induced pluripotent stem cells; Myocardial infarction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Cell Differentiation
  • Cell Proliferation
  • Cell-Free System
  • Extracellular Matrix / chemistry*
  • Fibroblasts / cytology
  • Fibroblasts / transplantation*
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / transplantation*
  • Male
  • Myocardial Infarction / pathology
  • Myocardial Infarction / therapy*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / transplantation
  • Organ Culture Techniques / instrumentation
  • Organ Culture Techniques / methods
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Engineering / instrumentation
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Treatment Outcome