Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model

J Vet Sci. 2017 Jun 30;18(2):245-251. doi: 10.4142/jvs.2017.18.2.245.


Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone.

Keywords: dog; doxycycline; horse chestnut leaf extract; matrix metalloproteinase; periodontal disease.

MeSH terms

  • Aesculus* / chemistry
  • Animals
  • Disease Models, Animal
  • Dog Diseases / drug therapy*
  • Dogs
  • Dose-Response Relationship, Drug
  • Gingiva / metabolism
  • Gingiva / surgery
  • Ligation / adverse effects
  • Ligation / veterinary
  • Matrix Metalloproteinase Inhibitors / therapeutic use*
  • Periodontitis / drug therapy
  • Periodontitis / veterinary*
  • Plant Extracts / administration & dosage
  • Plant Extracts / therapeutic use*
  • Plant Leaves* / chemistry


  • Matrix Metalloproteinase Inhibitors
  • Plant Extracts