CYP4Z1 3'UTR represses migration of human breast cancer cells

Biochem Biophys Res Commun. 2016 Sep 16;478(2):900-7. doi: 10.1016/j.bbrc.2016.08.048. Epub 2016 Aug 9.


To investigate the effects of CYP4Z1 3'UTR in migration of breast cancer cells, a series of assays were used to confirm that overexpression of CYP4Z1 3'UTR could suppress the capacity of migration and adhesion of MCF-7 and MDA-MB-231 cells. EMT (Epithelial-mesenchymal transition)-related proteins were regulated by CYP4Z1 3'UTR. Mesenchyma markers like Vimentin, MMP-2, and MMP-9 were down-regulated, while the expression of E-cadherin was up-regulated with CYP4Z1 3'UTR overexpression. Notably, luciferase reporter and qRT-PCR assays were applied to verify that CYP4Z1 3'UTR was the potential target of miR-9. In addition, our results showed that CYP4Z1 3'UTR repressed the expression of E-cadherin in a miRNA-dependent manner. Combining with our previous study, we have discovered the underlying link between CYP4Z1 and E-cadherin. Therefore, those preliminary data suggest that CYP4Z1 3'UTR could inhibit the migration and EMT of breast cancer cells via acting as a ceRNA for E-cadherin.

Keywords: Breast cancer; CYP4Z1; EMT; MicroRNA; Migration.

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Antigens, CD
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cadherins
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Cytochrome P450 Family 4 / genetics*
  • Cytochrome P450 Family 4 / metabolism
  • Epithelial-Mesenchymal Transition / genetics
  • Female
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • RNA, Small Interfering / metabolism
  • Transfection


  • 3' Untranslated Regions
  • Antigens, CD
  • CDH1 protein, human
  • Cadherins
  • MicroRNAs
  • RNA, Small Interfering
  • CYP4Z1 protein, human
  • Cytochrome P450 Family 4