Zika Virus-Induced Antibody Response Enhances Dengue Virus Serotype 2 Replication In Vitro

J Infect Dis. 2016 Nov 1;214(9):1357-1360. doi: 10.1093/infdis/jiw377. Epub 2016 Aug 11.


Zika virus has emerged in the Americas, where dengue virus is endemic. Among the 4 serotypes of dengue virus, antibody-dependent enhancement is thought to enhance viral replication and disease severity. Reports suggest that anti-dengue virus antibody may enhance Zika virus replication. We investigated whether Zika virus antibodies enhance dengue virus replication, by exposing C57Bl/6 mice to Zika virus. Polyclonal serum was verified for strong Zika virus-neutralizing, dengue virus-subneutralizing capacity. Then we determined the enhancement capabilities of Zika virus-immune serum for dengue virus in vitro. We showed that Zika virus antibodies have the ability to enhance dengue virus infections, which is important, because in many Zika virus-affected areas, dengue virus is expected to remain endemic.

Keywords: Zika; antibody; antibody dependent enhancement; dengue; enhancement; plaque reduction neutralization test (PRNT).

MeSH terms

  • Americas
  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology*
  • Antibody Formation / immunology*
  • Antibody-Dependent Enhancement / immunology*
  • Cell Line
  • Dengue / blood
  • Dengue / immunology
  • Dengue / virology
  • Dengue Virus / genetics*
  • Dengue Virus / immunology
  • Female
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Neutralization Tests / methods
  • Serogroup
  • Vero Cells
  • Viral Plaque Assay / methods
  • Virus Replication / genetics*
  • Zika Virus / immunology*
  • Zika Virus Infection / blood
  • Zika Virus Infection / immunology
  • Zika Virus Infection / virology


  • Antibodies, Neutralizing
  • Antibodies, Viral