Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 47 (4), 941-948

Lactobacillus Plantarum BL011 Cultivation in Industrial Isolated Soybean Protein Acid Residue

Affiliations

Lactobacillus Plantarum BL011 Cultivation in Industrial Isolated Soybean Protein Acid Residue

Chaline Caren Coghetto et al. Braz J Microbiol.

Abstract

In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87gL-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59gL-1, corresponding to a productivity of 1.46gL-1h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.

Keywords: Industrial production of biomass; Lactic acid; Lactobacillus plantarum BL011; Liquid acid protein residue of soybean; Plackett–Burman design.

Figures

Fig. 1
Fig. 1
Time course of batch cultivations of L. plantarum BL011 in medium containing (g L−1): MgSO4·7H2O, 0.2; MnSO4·H2O, 0.04; LAPRS, 40 (total sugars); yeast extract, 15. Culture conditions: 25 °C ± 1; 4.5 vvm; 200 rpm, pH 5.5 ± 0.2; (■) dry cell weight; (●) lactic acid concentration; (♦) acetic acid concentration; (▴) total sugars concentration. The results are the mean of duplicates.
Fig. 2
Fig. 2
Time course of batch cultivation of L. plantarum BL011 in hydrolysed LAPRS using invertase. Medium composition (g L−1): MgSO4·7H2O, 0.2; MnSO4·H2O, 0.04; LAPRS, 40 (total sugars); yeast extract, 15. Culture conditions: 25 °C ± 1; 4.5 vvm; 200 rpm, pH 5.5 ± 0.2; (■) dry cell weight; (●) lactic acid concentration; (♦) acetic acid concentration; (▴) total sugars concentration. The results are the mean of duplicates.
Fig. 3
Fig. 3
Time course of simultaneous saccharification and cultivation of L. plantarum BL011. Medium containing (g L−1): MgSO4·7H2O, 0.2; MnSO4·H2O, 0.04; LAPRS, 40 (total sugars); yeast extract, 15. Culture conditions: 25 °C ± 1; 4.5 vvm; 200 rpm, pH 5.5 ± 0.2 (■) dry cell weight; (●) lactic acid concentration; (♦) acetic acid concentration; (▴) total sugars concentration. The results are the mean of duplicates.
Fig. 4
Fig. 4
Time course of batch cultivation of L. plantarum BL011 under DOC of 30% saturation or higher. Medium composition (g L−1): MgSO4·7H2O, 0.2; MnSO4·H2O, 0.04; LAPRS, 40 (total sugars); yeast extract, 15. Culture conditions: 25 °C ± 1; 4.5 vvm; 200 rpm, pH 5.5 ± 0.2 (■) dry cell weight; (●) lactic acid concentration; (♦) acetic acid concentration; (▴) total sugars concentration. The results are the mean of duplicates.
Fig. 5
Fig. 5
Time course of batch cultivation of L. plantarum BL011 in hydrolysed LAPRS under DOC of 30% saturation or higher. Medium composition (g L−1): MgSO4·7H2O, 0.2; MnSO4·H2O, 0.04; LAPRS, 40 (total sugars); yeast extract, 15. Culture conditions: 25 °C ± 1; 4.5 vvm; 200 rpm, pH 5.5 ± 0.2; (■) dry cell weight; (●) lactic acid concentration; (♦) acetic acid concentration; (▴) total sugars concentration. The results are the mean of duplicates.

Similar articles

See all similar articles

Cited by 3 PubMed Central articles

References

    1. Brinques G.B., Peralba M.C., Ayub M.A.Z. Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems. J Ind Microbiol Biotechnol. 2010;37:205–212. - PubMed
    1. Li Z., Han L., Ji Y., Wang X., Tan T. Fermentative production of l-lactic acid from hydrolysate of wheat bran by Lactobacillus rhamnosus. Biochem Eng J. 2010;49:138–142.
    1. Silva G.A.B., Almeida W.E.S., Cortes M.S., Martins E.S. Production and characterization of protease produced by Gliocladium verticilloides by solid state fermentation of agroindustrial by-products. Braz J Agroind Technol. 2009;3:28–41.
    1. Srisamai S., Srikhampa P., Pathom-Aree W. Production of probiotic streptomyces biomass from starchy wastewater. Chiang Mai J Sci. 2013;40:294–298.
    1. Wee Y.J., Ryu H.W. Lactic acid production by Lactobacillus sp. RKY2 in a cell-recycle continuous fermentation using lignocellulosic hydrolyzates as inexpensive raw materials. Bioresour Technol. 2009;100:4262–4270. - PubMed

LinkOut - more resources

Feedback