Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Multicenter Study
. 2016 Oct-Dec;47(4):925-930.
doi: 10.1016/j.bjm.2016.07.016. Epub 2016 Jul 26.

The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran

Roghayeh Nouri  1 , Mohammad Ahangarzadeh Rezaee  2 , Alka Hasani  3 , Mohammad Aghazadeh  4 , Mohammad Asgharzadeh  5
Affiliations
Multicenter Study

The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran

Roghayeh Nouri et al. Braz J Microbiol. 2016 Oct-Dec.

Abstract

The aim of this study was to examine mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes in Pseudomonas aeruginosa isolates. A total of 100 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz, Iran. Minimum inhibitory concentrations (MICs) of ciprofloxacin and levofloxacin were evaluated by agar dilution assay. DNA sequences of the QRDR of gyrA and parC were determined by the dideoxy chain termination method. Of the total 100 isolates, 64 were resistant to ciprofloxacin. No amino acid alterations were detected in gyrA or parC genes of the ciprofloxacin susceptible or ciprofloxacin intermediate isolates. Thr-83 → Ile substitution in gyrA was found in all 64 ciprofloxacin resistant isolates. Forty-four (68.75%) of them had additional substitution in parC. A correlation was found between the number of the amino acid alterations in the QRDR of gyrA and parC and the level of ciprofloxacin and levofloxacin resistance of the P. aeruginosa isolates. Ala-88 → Pro alteration in parC was generally found in high level ciprofloxacin resistant isolates, which were suggested to be responsible for fluoroquinolone resistance. These findings showed that in P. aeruginosa, gyrA was the primary target for fluoroquinolone and additional mutation in parC led to highly resistant isolates.

Keywords: Fluoroquinolone resistance; Pseudomonas aeruginosa; gyrA; parC.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Ahangarzadeh Rezaee M., Behzadiannezhad Q., Najjar-Pirayeh S., Oulia P. In vitro activity of imipenem and ceftazidime against mucoid and non-mucoid strains of Pseudomonas aeruginosa isolated from patients in Iran. Arch Iran Med. 2002;5:251–254.
    1. Lihua L., Jianhuit W., Jialini Y., Yayin L., Guanxin L. Effects of allicin on the formation of Pseudomonas aeruginosa biofinm and the production of quorum-sensing controlled virulence factors. Pol J Microbiol. 2013;62:243–251. - PubMed
    1. Wolska K., Szweda P. Genetic features of clinical Pseudomonas aeruginosa strains. Pol J Microbiol. 2009;58:255–260. - PubMed
    1. Ahangarzadeh Rezaee M., Behzadiannezhad Q., Najjar P.S., Oulia P. Higher aminoglycoside resistance in mucoid Pseudomonas aeruginosa than in non-mucoid strains. Arch Iranian Med. 2002;5:108–110.
    1. Oliveira ACd., Maluta R.P., Stella A.E., Rigobelo E.C., Marin J.M., Ávila FAd. Isolation of Pseudomonas aeruginosa strains from dental office environments and units in Barretos, state of São Paulo, Brazil, and analysis of their susceptibility to antimicrobial drugs. Braz J Microbiol. 2008;39:579–584. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources