Siglec-F is a novel intestinal M cell marker

Biochem Biophys Res Commun. 2016 Oct 7;479(1):1-4. doi: 10.1016/j.bbrc.2016.08.055. Epub 2016 Aug 11.

Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.

Keywords: M cell; Oral vaccine; Peyer's patch; Siglec.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Antibody Affinity / immunology
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Antigens, Differentiation, Myelomonocytic / immunology
  • Antigens, Differentiation, Myelomonocytic / metabolism*
  • Biomarkers / metabolism*
  • CHO Cells
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cricetinae
  • Cricetulus
  • Flow Cytometry
  • HEK293 Cells
  • Humans
  • Immunohistochemistry
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / metabolism*
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology
  • Peyer's Patches / metabolism*
  • Protein Binding / immunology
  • Rats, Inbred Lew

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Biomarkers
  • Siglecf protein, mouse