Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin

Christopher A Baker; Yishai M Elyada; Andres Parra; M McLean Bolton

doi:10.7554/eLife.14193

Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin

Elife. 2016 Aug 15:5:e14193. doi: 10.7554/eLife.14193.

Authors

Christopher A Baker¹, Yishai M Elyada², Andres Parra², M McLean Bolton¹

Affiliations

¹ Disorders of Neural Circuit Function, Max Planck Florida Institute for Neuroscience, Jupiter, United States.
² Functional Architecture of the Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, United States.

Abstract

We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.

Keywords: circuit; connectivity; mouse; neuroscience; optogenetics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Action Potentials
Animals
Brain Mapping / methods*
Mice, Inbred C57BL
Nerve Net*
Optogenetics / methods*
Patch-Clamp Techniques / methods*

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.