DSBCapture: in situ capture and sequencing of DNA breaks

Nat Methods. 2016 Oct;13(10):855-7. doi: 10.1038/nmeth.3960. Epub 2016 Aug 15.


Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Chromatin / metabolism
  • DNA / genetics*
  • DNA Breaks, Double-Stranded*
  • DNA End-Joining Repair / genetics
  • Epigenesis, Genetic
  • HeLa Cells
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Keratinocytes / metabolism
  • Keratinocytes / pathology
  • Sensitivity and Specificity
  • Sequence Analysis, DNA


  • Chromatin
  • DNA