Multi-modality Optical Imaging of Rat Kidney Dysfunction: In Vivo Response to Various Ischemia Times

Adv Exp Med Biol. 2016;923:345-350. doi: 10.1007/978-3-319-38810-6_45.


We observed in vivo kidney dysfunction with various ischemia times at 30, 75, 90, and 120 min using multi-modality optical imaging: optical coherence tomography (OCT), Doppler OCT (DOCT), and two-photon microscopy (TPM). We imaged the renal tubule lumens and glomerulus at several areas of each kidney before, during, and after ischemia of 5-month-old female Munich-Wistar rats. For animals with 30 and 75 min ischemia times, we observed that all areas were recovered after ischemia, that tubule lumens were re-opened and the blood flow of the glomerulus was re-established. For animals with 90 and 120 min ischemia times, we observed unrecovered areas, and that tubule lumens remained close after ischemia. TPM imaging verified the results of OCT and provided higher resolution images than OCT to visualize renal tubule lumens and glomerulus blood flow at the cellular level.

Keywords: Doppler optical coherence tomography; Ischemia; Kidney dysfunction; Optical coherence tomography; Two-photon microscopy.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Acute Kidney Injury / diagnostic imaging*
  • Acute Kidney Injury / physiopathology
  • Animals
  • Disease Models, Animal
  • Female
  • Fourier Analysis
  • Kidney Glomerulus / blood supply
  • Kidney Glomerulus / diagnostic imaging*
  • Kidney Tubules / diagnostic imaging*
  • Kidney Tubules / physiopathology
  • Microscopy, Fluorescence, Multiphoton*
  • Multimodal Imaging / methods*
  • Predictive Value of Tests
  • Rats, Wistar
  • Recovery of Function
  • Renal Circulation
  • Reperfusion Injury / diagnostic imaging*
  • Reperfusion Injury / physiopathology
  • Time Factors
  • Tomography, Optical Coherence*
  • Ultrasonography, Doppler*