Correlation of Checkerboard Synergy Testing with Time-Kill Analysis and Clinical Outcomes of Extensively Drug-Resistant Acinetobacter baumannii Respiratory Infections

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6892-6895. doi: 10.1128/AAC.00981-16. Print 2016 Nov.

Abstract

We tested 76 extensively drug-resistant (XDR) Acinetobacter baumannii isolates by the checkerboard method using only wells containing serum-achievable concentrations (SACs) of drugs. Checkerboard results were correlated by time-kill assay and clinical outcomes. Minocycline-colistin was the best combination in vitro, as it inhibited growth in one or more SAC wells in all isolates. Patients who received a combination that inhibited growth in one or more SAC wells demonstrated better microbiological clearance than those who did not (88% versus 30%; P = 0.025). The checkerboard platform may have clinical utility for XDR A. baumannii infections.

Publication types

  • Clinical Trial

MeSH terms

  • Acinetobacter Infections / drug therapy*
  • Acinetobacter Infections / microbiology
  • Acinetobacter baumannii / drug effects*
  • Acinetobacter baumannii / pathogenicity
  • Anti-Bacterial Agents / pharmacology*
  • Bacteremia / drug therapy
  • Bacteremia / microbiology
  • Colistin / pharmacology
  • Drug Resistance, Bacterial / drug effects
  • Drug Synergism
  • Drug Therapy, Combination
  • Female
  • Humans
  • Male
  • Microbial Sensitivity Tests / methods*
  • Middle Aged
  • Minocycline / pharmacology
  • Pneumonia, Bacterial / drug therapy
  • Pneumonia, Bacterial / microbiology
  • Respiratory Tract Infections / drug therapy*
  • Respiratory Tract Infections / microbiology
  • Treatment Outcome

Substances

  • Anti-Bacterial Agents
  • Minocycline
  • Colistin

Grants and funding

There was no financial support for this work.