Abstract
We tested 76 extensively drug-resistant (XDR) Acinetobacter baumannii isolates by the checkerboard method using only wells containing serum-achievable concentrations (SACs) of drugs. Checkerboard results were correlated by time-kill assay and clinical outcomes. Minocycline-colistin was the best combination in vitro, as it inhibited growth in one or more SAC wells in all isolates. Patients who received a combination that inhibited growth in one or more SAC wells demonstrated better microbiological clearance than those who did not (88% versus 30%; P = 0.025). The checkerboard platform may have clinical utility for XDR A. baumannii infections.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
MeSH terms
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Acinetobacter Infections / drug therapy*
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Acinetobacter Infections / microbiology
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Acinetobacter baumannii / drug effects*
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Acinetobacter baumannii / pathogenicity
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Anti-Bacterial Agents / pharmacology*
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Bacteremia / drug therapy
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Bacteremia / microbiology
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Colistin / pharmacology
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Drug Resistance, Bacterial / drug effects
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Drug Synergism
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Drug Therapy, Combination
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Female
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Humans
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Male
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Microbial Sensitivity Tests / methods*
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Middle Aged
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Minocycline / pharmacology
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Pneumonia, Bacterial / drug therapy
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Pneumonia, Bacterial / microbiology
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Respiratory Tract Infections / drug therapy*
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Respiratory Tract Infections / microbiology
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Treatment Outcome
Substances
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Anti-Bacterial Agents
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Minocycline
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Colistin
Grants and funding
There was no financial support for this work.