Overexpression of the Eggplant (Solanum melongena) NAC Family Transcription Factor SmNAC Suppresses Resistance to Bacterial Wilt

Sci Rep. 2016 Aug 16;6:31568. doi: 10.1038/srep31568.

Abstract

Bacterial wilt (BW) is a serious disease that affects eggplant (Solanum melongena) production. Although resistance to this disease has been reported, the underlying mechanism is unknown. In this study, we identified a NAC family transcription factor (SmNAC) from eggplant and characterized its expression, its localization at the tissue and subcellular levels, and its role in BW resistance. To this end, transgenic eggplant lines were generated in which the expression of SmNAC was constitutively up regulated or suppressed using RNAi. The results indicated that overexpression of SmNAC decreases resistance to BW. Moreover, SmNAC overexpression resulted in the reduced accumulation of the plant immune signaling molecule salicylic acid (SA) and reduced expression of ICS1 (a gene that encode isochorismate synthase 1, which is involved in SA biosynthesis). We propose that reduced SA content results in increased bacterial wilt susceptibility in the transgenic lines. Our results provide important new insights into the regulatory mechanisms of bacterial wilt resistance in eggplant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Genes, Plant
  • Immunohistochemistry
  • Plant Diseases / immunology*
  • Plant Diseases / microbiology
  • Plants, Genetically Modified
  • Promoter Regions, Genetic
  • Ralstonia solanacearum / pathogenicity*
  • Sequence Analysis, RNA
  • Sequence Homology, Amino Acid
  • Solanum melongena / genetics*
  • Solanum melongena / immunology
  • Solanum melongena / microbiology
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*

Substances

  • Transcription Factors