The main objective of this study was the development of simple multi-parameter methods for the analyses of biocides in various environmental matrices (wastewater, surface water, and sewage sludge) for measurement and monitoring activities. Eight target substances (triclosan, methyltriclosan (transformation product of triclosan), cybutryne (Irgarol), and the azole fungicides propiconazole, tebuconazole, imazalil, thiabendazole, and cyproconazole) were chosen for determination in selected sample sets. For surface water and wastewater samples a solid-phase extraction (SPE) method and for sewage sludge samples an accelerated solvent extraction (ASE) were developed. The extracts were analyzed by gas chromatographic-mass spectrometric methods (GC/MS), and the analytical methods were checked to ensure sufficient sensitivity by comparing the limits of quantification (LOQs) to the predicted no effect concentrations (PNECs) of the selected biocides. For quality control, recovery rates were determined. Finally, developed methods were checked and validated by application on sample material from various matrices. Sampling took place in seven urban wastewater treatment plants and their corresponding receiving waters. The results revealed that the developed extraction methods are effective and simple and allow the determination of a broad range of biocides in various environmental compartments.
Keywords: Accelerated solvent extraction; Azole fungicides; Biocides; Environmental compartments; GC/MS analysis; Multi-parameter method; Solid-phase extraction; Wastewater.