Cloning and Functional Analysis of Three Chalcone Synthases from the Flowers of Safflowers Carthamus tinctorius
- PMID: 27534116
Cloning and Functional Analysis of Three Chalcone Synthases from the Flowers of Safflowers Carthamus tinctorius
Abstract
The flowers of safflowers (Carthamus tinctorius L.) are very important as they are the sole source of their distinct pigments, i.e. carthamus-red and -yellows, and have historically had strong connections to the cultural side of human activities such as natural dyes, rouge, and traditional medicines. The distinct pigments are quinochalcone C-glucosides, which are found specifically in the flowers of C. tinctorius. To investigate the biosynthetic pathways of quinochalcone C-glucosides, de novo assembly of the transcriptome was performed on the flowers using an Illumina sequencing platform to obtain 69,312 annotated coding DNA sequences. Three chalcone synthase like genes, CtCHS1, 2 and 3 were focused on and cloned, which might be involved in quinochalcone C-glucosides biosynthesis by establishing the C6-C3-C6 chalcone skeleton. It was demonstrated that all the recombinant CtCHSs could recognize p-coumaroyl-CoA, caffeoyl-CoA, feruloyl-CoA, and sinapoyl-CoA as starter substrates. This is the first report on the cloning and functional analysis of the three chalcone synthase genes from the flowers of C. tinctorius.
Similar articles
-
Cloning, expression and activity analysises of chalcone synthase genes in Carthamus tinctorius.Chin Herb Med. 2023 Feb 16;15(2):291-297. doi: 10.1016/j.chmed.2022.12.005. eCollection 2023 Apr. Chin Herb Med. 2023. PMID: 37265765 Free PMC article.
-
Identification of the Genes Coding for Carthamin Synthase, Peroxidase Homologs that Catalyze the Final Enzymatic Step of Red Pigmentation in Safflower (Carthamus tinctorius L.).Plant Cell Physiol. 2021 Dec 3;62(10):1528-1541. doi: 10.1093/pcp/pcab122. Plant Cell Physiol. 2021. PMID: 34343331
-
[Full-length cDNA cloning of flavonol synthase genes of Carthamus tinctorius and construction plant expression vector].Zhongguo Zhong Yao Za Zhi. 2015 Feb;40(4):634-8. Zhongguo Zhong Yao Za Zhi. 2015. PMID: 26137682 Chinese.
-
Phytochemistry and Pharmacology of Carthamus tinctorius L.Am J Chin Med. 2016;44(2):197-226. doi: 10.1142/S0192415X16500130. Am J Chin Med. 2016. PMID: 27080938 Review.
-
Chemical and biological properties of quinochalcone C-glycosides from the florets of Carthamus tinctorius.Molecules. 2013 Dec 10;18(12):15220-54. doi: 10.3390/molecules181215220. Molecules. 2013. PMID: 24335575 Free PMC article. Review.
Cited by
-
Integrated metabolomics and transcriptome analysis on flavonoid biosynthesis in safflower (Carthamus tinctorius L.) under MeJA treatment.BMC Plant Biol. 2020 Jul 29;20(1):353. doi: 10.1186/s12870-020-02554-6. BMC Plant Biol. 2020. PMID: 32727365 Free PMC article.
-
Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis in Carthamus tinctorius L.J Agric Food Chem. 2023 Nov 1;71(43):16221-16232. doi: 10.1021/acs.jafc.3c05117. Epub 2023 Oct 23. J Agric Food Chem. 2023. PMID: 37870279 Free PMC article.
-
Cloning, expression and activity analysises of chalcone synthase genes in Carthamus tinctorius.Chin Herb Med. 2023 Feb 16;15(2):291-297. doi: 10.1016/j.chmed.2022.12.005. eCollection 2023 Apr. Chin Herb Med. 2023. PMID: 37265765 Free PMC article.
-
Phylogenetic relationship and sequence diversity of candidate genes involved in anthocyanin biosynthesis pathway in Carthamus species with contrasting seed coat colors.Physiol Mol Biol Plants. 2023 Mar;29(3):349-360. doi: 10.1007/s12298-023-01298-5. Epub 2023 Mar 22. Physiol Mol Biol Plants. 2023. PMID: 37033761 Free PMC article.
-
Overexpression of CtCHS1 Increases Accumulation of Quinochalcone in Safflower.Front Plant Sci. 2017 Aug 15;8:1409. doi: 10.3389/fpls.2017.01409. eCollection 2017. Front Plant Sci. 2017. PMID: 28861095 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
LinkOut - more resources
Miscellaneous