Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases

J Biol Chem. 2016 Oct 7;291(41):21607-21615. doi: 10.1074/jbc.M116.728006. Epub 2016 Aug 18.


The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms.

Keywords: Aspergillus; Rid/YjgF/YER057c/UK114 family; allergen; allergy; asthma; fungi; house dust mite; imine deaminase; immunoglobulin E (IgE).

MeSH terms

  • Aminohydrolases* / genetics
  • Aminohydrolases* / immunology
  • Animals
  • Antigens, Dermatophagoides* / genetics
  • Antigens, Dermatophagoides* / immunology
  • Arthropod Proteins* / genetics
  • Arthropod Proteins* / immunology
  • Aspergillus fumigatus / genetics
  • Aspergillus fumigatus / immunology
  • Cross Reactions
  • Dermatophagoides farinae* / genetics
  • Dermatophagoides farinae* / immunology
  • Female
  • Fungal Proteins / genetics
  • Fungal Proteins / immunology
  • Humans
  • Male


  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Fungal Proteins
  • Aminohydrolases