Discovery of a proteinaceous cellular receptor for a norovirus

Science. 2016 Aug 26;353(6302):933-6. doi: 10.1126/science.aaf1220. Epub 2016 Aug 18.


Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host molecules that are essential for murine NoV (MNoV)-induced cell death, including CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV binding and replication in cell lines and primary cells. Additionally, Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in human cells breaks the species barrier that would otherwise restrict MNoV replication. The crystal structure of the CD300lf ectodomain reveals a potential ligand-binding cleft composed of residues that are critical for MNoV infection. Therefore, the presence of a proteinaceous receptor is the primary determinant of MNoV species tropism, whereas other components of cellular machinery required for NoV replication are conserved between humans and mice.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Caliciviridae Infections / virology*
  • Crystallography, X-Ray
  • Gastroenteritis / virology*
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Mutant Strains
  • Norovirus / physiology*
  • Protein Domains
  • Receptors, Immunologic* / chemistry
  • Receptors, Immunologic* / genetics
  • Receptors, Immunologic* / metabolism
  • Virus Internalization*
  • Virus Replication*


  • CLM-1 protein, mouse
  • Receptors, Immunologic