Genomic and proteomic characterization of SE-I, a temperate bacteriophage infecting Erysipelothrix rhusiopathiae

Arch Virol. 2016 Nov;161(11):3137-50. doi: 10.1007/s00705-016-3018-1. Epub 2016 Aug 19.


A bacteriophage infecting pathogenic Erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage SE-I. SE-I has an icosahedral head, a long tail and a double-stranded DNA genome. The 34,997-bp genome has a GC content of 34 % and contains 43 open reading frames (ORFs) encoding packaging, structural, lysin-holin, and hypothetical proteins. Components of purified SE-I were separated using SDS-PAGE and analyzed using liquid chromatography-mass spectrometry. Nine proteins were identified, encoded by ORF9, ORF15, ORF23, ORF30, ORF31, ORF33, ORF39, ORF40 and ORF 42. A phylogenetic tree constructed based on the sequence of the large terminase subunit revealed that SE-I is closely related to Staphylococcus phages P954 and phi3396. The CHAP-domain-containing protein encoded by ORF25 was expressed in E. coli and which was able to inactivate host bacteria. SE-I was able to infect 7 of 13 E. rhusiopathiae strains, but was unable to infect Salmonella, Streptococcus suis, and Staphylococcus aureus. This is the first report of the isolation, characterization, and genomic and proteomic analysis of a temperate phage infecting E. rhusiopathiae, and it might lead to the development of new anti- E. rhusiopathiae agents.

MeSH terms

  • Animals
  • Bacteriolysis
  • Bacteriophages / chemistry
  • Bacteriophages / genetics
  • Bacteriophages / isolation & purification*
  • Bacteriophages / ultrastructure
  • Base Composition
  • Chromatography, Liquid
  • Cluster Analysis
  • DNA / chemistry
  • DNA / genetics
  • DNA, Viral / chemistry
  • DNA, Viral / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Erysipelas / microbiology
  • Erysipelas / veterinary
  • Erysipelothrix / virology*
  • Escherichia coli
  • Farms
  • Gene Order
  • Genome, Viral*
  • Genomics
  • Host Specificity
  • Mass Spectrometry
  • Open Reading Frames
  • Phylogeny
  • Proteomics
  • Sequence Analysis, DNA
  • Sequence Homology
  • Staphylococcus aureus
  • Streptococcus suis
  • Swine
  • Viral Proteins / analysis*
  • Virion / ultrastructure


  • DNA, Viral
  • Viral Proteins
  • DNA