Flexible analysis of digital PCR experiments using generalized linear mixed models

Biomol Detect Quantif. 2016 Jun 24;9:1-13. doi: 10.1016/j.bdq.2016.06.001. eCollection 2016 Sep.

Abstract

The use of digital PCR for quantification of nucleic acids is rapidly growing. A major drawback remains the lack of flexible data analysis tools. Published analysis approaches are either tailored to specific problem settings or fail to take into account sources of variability. We propose the generalized linear mixed models framework as a flexible tool for analyzing a wide range of experiments. We also introduce a method for estimating reference gene stability to improve accuracy and precision of copy number and relative expression estimates. We demonstrate the usefulness of the methodology on a complex experimental setup.

Keywords: Data analysis; Digital PCR; Mixed models; Quantification; Replicates; Statistics.