Longitudinal diffusion tensor imaging of the rat brain after hexachlorophene exposure

Neurotoxicology. 2016 Sep:56:225-232. doi: 10.1016/j.neuro.2016.08.011. Epub 2016 Aug 20.


Longitudinal MRI employing diffusion tensor imaging and T2 mapping approaches has been applied to investigate the mechanisms of white matter damage caused by acute hexachlorophene neurotoxicity in rats in vivo. Male Sprague-Dawley rats were administered hexachlorophene orally once a day for five consecutive days at a dose of 30mg/kg and were monitored in 7T MRI scanner at days 0 (baseline), 3, 6, 13, and 20 following the first hexachlorophene dose. Quantitative T2 maps as well as a number of diffusion tensor parameters (fractional anisotropy, radial and axial diffusivity, apparent diffusion coefficient, and trace) were calculated from corresponding MR images. T2, as well as all diffusion tensor derived parameters (except fractional anisotropy) showed significant changes during the course of neurotoxicity development. These changes peaked at 6days after the first dose of hexachlorophene (one day after the last dose) and recovered to practically baseline levels at the end of observation (20days from the first dose). While such changes in diffusivity and T2 relaxation clearly demonstrate myelin perturbations consistent with edema, the lack of changes of fractional anisotropy suggests that the structure of the myelin sheath was not disrupted significantly by hexachlorophene in this study. This is also confirmed by the rapid recovery of all observed MRI parameters after cessation of hexachlorophene exposure.

Keywords: Axial diffusivity; DTI; Fractional anisotropy; Hexachlorophene; Radial diffusivity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anisotropy
  • Anti-Infective Agents, Local / pharmacology*
  • Brain / diagnostic imaging*
  • Brain / drug effects*
  • Diffusion Tensor Imaging*
  • Hexachlorophene / pharmacology*
  • Image Processing, Computer-Assisted
  • Longitudinal Studies
  • Male
  • Nerve Fibers, Myelinated / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors
  • White Matter / diagnostic imaging
  • White Matter / drug effects


  • Anti-Infective Agents, Local
  • Hexachlorophene