A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules

Methods Mol Biol. 2016:1482:175-88. doi: 10.1007/978-1-4939-6396-6_12.


Transcription factors often form protein complexes and give rise to intricate transcriptional networks. The regulation of transcription factor multimerization plays a key role in the fine-tuning of the underlying transcriptional pathways and can be exploited to modulate synthetic transcriptional modules. A novel regulation of protein complex formation is emerging: microProteins-truncated transcription factors-engage in protein-protein interactions with transcriptional complexes and modulate their transcriptional activity. Here, we outline a strategy for the discovery, design, and test of putative miPs to fine-tune the activity of transcription factors regulating synthetic or natural transcriptional circuits.

Keywords: Multimerization; Protein complex; Protein–protein interaction domain; Synthetic promoter; Transcription factor; microProteins.

MeSH terms

  • Gene Expression Regulation / genetics*
  • Gene Regulatory Networks / genetics
  • Molecular Biology / methods*
  • Protein Interaction Maps / genetics
  • Proteins / genetics*
  • Transcription Factors / genetics
  • Transcription, Genetic*


  • Proteins
  • Transcription Factors