Predominance of Th1 Response, Increase of Megakaryocytes and Kupffer Cells Are Related to Survival in Trypanosoma Cruzi Infected Mice Treated With Lycopodium Clavatum

Cytokine. 2016 Dec;88:57-61. doi: 10.1016/j.cyto.2016.08.015. Epub 2016 Aug 24.

Abstract

We investigated the number of megakaryocytes, Kupffer cells and ratios of Th1/Th2 and Th1/Th17 cytokines in survival of mice infected with Y strain of Trypanosoma cruzi and treated with Lycopodium clavatum. In a blind, randomized and controlled assay, Swiss male mice, 8weeks-old, infected with 1400 trypomastigotes (Y strain) were divided into groups and treated with: GLy - Lycopodium clavatum dynamization13c and GCI - alcohol solution 7° GL (vehicle medicine). The treatment was offered two days before infection and on the 2nd, 4th and 6th days after infection, overnight (1mL/100mL) and ad libitum. Parameters assessed were: survival rate, number of megakaryocytes and Kupffer cells, cytokines dosage (TNF-α, IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17), Th1/Th2 and Th1/Th17 ratios. The increase in megakaryocytes, Kupffer cells, predominance of Th1 response, with increased TNF-α, IL-10, TNF-α/IL-4, TNF-α/IL-17 and decreased IL-6 IL-6/IL-4, are related to increased survival in mice infected with T. cruzi and treated with Lycopodium clavatum 13c. This result demonstrates the possibility of an alternative approach for the treatment of Chagas disease with dynamized drugs.

Keywords: Kupffer cells; Lycopodium clavatum; Megakaryocytes; Th1 response; Trypanosoma cruzi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chagas Disease / drug therapy
  • Chagas Disease / metabolism*
  • Cytokines / metabolism*
  • Kupffer Cells / metabolism*
  • Lycopodium*
  • Male
  • Megakaryocytes / metabolism*
  • Mice
  • Th1 Cells / metabolism*
  • Trypanosoma cruzi / metabolism*

Substances

  • Cytokines