Bacterioplanoides pacificum gen. nov., sp. nov., isolated from seawater of South Pacific Gyre

Int J Syst Evol Microbiol. 2016 Dec;66(12):5010-5015. doi: 10.1099/ijsem.0.001461. Epub 2016 Aug 26.

Abstract

A Gram-staining-negative, strictly aerobic, transparent and smooth, curved-rod-shaped motile bacterium with a single polar flagellum, designated strain sw100T, was isolated from surface seawater of South Pacific Gyre during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sw100T was most closely related to the member of the family Oceanospirillaceae and was distinct from the most closely related genera Bacterioplanes, Thalassolituus and Oceanobacter (95.8, 94.5-95.3 and 94.4 % 16S rRNA gene sequence similarities, respectively). Growth occurred in the presence of 0-11 % NaCl (w/v, optimum 7 %), at 10-37 °C (optimum 28 °C) and pH 6.0-9.0 (optimum pH 7.0). The major fatty acids (>10 % of total fatty acids) are C16 : 0, C16 : 0 N alcohol and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. The DNA G+C content of strain sw100T was 55.3 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). On the basis of polyphasic analyses, strain sw100T is considered to represent a novel species in a novel genus of the family Oceanospirillaceae, for which the name Bacterioplanoides pacificum gen. nov., sp. nov. is proposed. The type strain is sw100T (=JCM 30597T=MCCC 1K00501T=KCTC 42424T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Oceanospirillaceae / classification*
  • Oceanospirillaceae / genetics
  • Oceanospirillaceae / isolation & purification
  • Pacific Ocean
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / chemistry
  • Water Microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • Ubiquinone Q2