Efficient transformation of Staphylococcus aureus using multi-pulse electroporation

Junzo Hisatsune; Yusuke Sato'o; Liansheng Yu; Shoko Kutsuno; Yasuhiko Hayakawa; Motoyuki Sugai

doi:10.1016/j.mimet.2016.08.012

Efficient transformation of Staphylococcus aureus using multi-pulse electroporation

J Microbiol Methods. 2016 Nov:130:69-72. doi: 10.1016/j.mimet.2016.08.012. Epub 2016 Aug 24.

Authors

Junzo Hisatsune¹, Yusuke Sato'o¹, Liansheng Yu¹, Shoko Kutsuno¹, Yasuhiko Hayakawa², Motoyuki Sugai³

Affiliations

¹ Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.
² Nepa Gene Co., Ltd., Chiba, Japan.
³ Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan. Electronic address: sugai@hiroshima-u.ac.jp.

PMID: 27567890
DOI: 10.1016/j.mimet.2016.08.012

Abstract

A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9×10⁵S. aureus RN4220 transformed cells/1μg plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse.

Keywords: Competency; ELEPO21; Multi-pulse electroporation; Plasmid DNA; Poring pulse; Staphylococcus aureus; Transfer pulse; Transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial / genetics
Electric Conductivity
Electricity
Electroporation / instrumentation*
Electroporation / methods*
Genetic Techniques / instrumentation
Plasmids
Staphylococcus aureus / genetics*
Temperature
Transformation, Bacterial*

Substances

DNA, Bacterial